.: ORİJİNAL ARAŞTIRMA
Kompozit Materyallerin Gingival Fibroblast Hücreleri Üzerindeki Sitotoksik Etkisinin İncelenmesi
Investigation of the Cytotoxic Effect of Composite Materials on Gingival Fibroblast Cells
Turkiye Klinikleri J Dental Sci. 2019;25(3):310-8
doi: 10.5336/dentalsci.2018-63270
Makale Dili: TR
Tam Metin
ÖZET
Amaç: Oral dokularla temas eden restoratif materyaller bu dokularda inflamatuar, alerjik, toksik, mutajenik ve karsinojenik reaksiyonlara neden olabilmektedirler. Bu nedenle biyouyumluluk restoratif materyallerde ön planda tutulması gereken bir faktördür. Bu çalışmada rezin içerikli dolgu materyallerinin, dişeti fibroblast kök hücrelerine sitotoksik etkilerinin 3-(4,5-dimetiltiyazol- 2-yl)-2,5-difeniltetrazolyum bromür (MTT) analizi ile değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: Çalışmada, yeni nesil (bir adet bulk fill, bir adet supra-nanohibrid, iki adet nanohibrid, iki adet mikrohibrid) altı farklı kompozit materyal kullanıldı. Çalışma grupları; XF: X-tra Fill (Voco- Almanya), GA: G- ænial Posterior (GC Tokyo Japonya), ES: Estelite Sigma Quick (Tokuyama-Japonya), GO: Grandio (Voco-Almanya), AB: Arabesk (Voco-Almanya) ve PS: Polofil Supra (Voco-Almanya) olarak hazırlandı. Her materyal için örnek sayısı 12 olarak belirlendi (n=12). Örnekler teflon kalıplar kullanılarak hazırlandı. GFBCs'lerin 72 saat süreyle örneklerle teması sonucu hücre canlılık oranları MTT analiziyle değerlendirildi. Bulgular: Grupların hücre canlılık oranları (%) sırasıyla; PS>AB>GO>ES>XF>GA olarak saptanmıştır. Sonuç: Bir materyalin sitotoksisitesinde; materyalin yapısı, içerdiği (doldurucu) oranı, monomer tipi, doldurucu içeriği gibi faktörlerin bir bütün olarak etkili olduğu, trietilen glikol dimetakrilat, etilen glikol dimetakrilat monomer varlığının materyalin potansiyel toksisite olasılığını artıran monomerler olduğu, doldurucu içeriğine eklenen parçacıkların (Fluro-alüminosilikat partikülleri, iterium trifluoride parçacıkları vb.) sitotoksisiteyi etkileyebileceği sonucuna varılmıştır.
Anahtar Kelimeler: Kompozit rezin; kök hücre; hücre canlılığı; sitotoksisite; MTT analizi
Amaç: Oral dokularla temas eden restoratif materyaller bu dokularda inflamatuar, alerjik, toksik, mutajenik ve karsinojenik reaksiyonlara neden olabilmektedirler. Bu nedenle biyouyumluluk restoratif materyallerde ön planda tutulması gereken bir faktördür. Bu çalışmada rezin içerikli dolgu materyallerinin, dişeti fibroblast kök hücrelerine sitotoksik etkilerinin 3-(4,5-dimetiltiyazol- 2-yl)-2,5-difeniltetrazolyum bromür (MTT) analizi ile değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: Çalışmada, yeni nesil (bir adet bulk fill, bir adet supra-nanohibrid, iki adet nanohibrid, iki adet mikrohibrid) altı farklı kompozit materyal kullanıldı. Çalışma grupları; XF: X-tra Fill (Voco- Almanya), GA: G- ænial Posterior (GC Tokyo Japonya), ES: Estelite Sigma Quick (Tokuyama-Japonya), GO: Grandio (Voco-Almanya), AB: Arabesk (Voco-Almanya) ve PS: Polofil Supra (Voco-Almanya) olarak hazırlandı. Her materyal için örnek sayısı 12 olarak belirlendi (n=12). Örnekler teflon kalıplar kullanılarak hazırlandı. GFBCs'lerin 72 saat süreyle örneklerle teması sonucu hücre canlılık oranları MTT analiziyle değerlendirildi. Bulgular: Grupların hücre canlılık oranları (%) sırasıyla; PS>AB>GO>ES>XF>GA olarak saptanmıştır. Sonuç: Bir materyalin sitotoksisitesinde; materyalin yapısı, içerdiği (doldurucu) oranı, monomer tipi, doldurucu içeriği gibi faktörlerin bir bütün olarak etkili olduğu, trietilen glikol dimetakrilat, etilen glikol dimetakrilat monomer varlığının materyalin potansiyel toksisite olasılığını artıran monomerler olduğu, doldurucu içeriğine eklenen parçacıkların (Fluro-alüminosilikat partikülleri, iterium trifluoride parçacıkları vb.) sitotoksisiteyi etkileyebileceği sonucuna varılmıştır.
Anahtar Kelimeler: Kompozit rezin; kök hücre; hücre canlılığı; sitotoksisite; MTT analizi
ABSTRACT
Objective: Restorative materials in contact with oral tissues may cause inflammatory, allergic, toxic, mutagenic and carcinogenic reactions in these tissues. Therefore, biocompatibility factor is a factor that must be kept in foreground in restorative materials. The aim of this study was to evaluate the cytotoxic effects of resin-containing filler materials on gingival fibroblast stem cells by 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) analysis. Material and Methods: Six different composite materials of the new generation (one bulk fill, one supra-nanohybrid, two nanohybrid, two micro-hybrid) were used in the study. The study groups were prepared as XF: X-tra Fill (Voco-Germany), GA: G-anial Posterior (GC Tokyo Japan), ES: Estelite Sigma Quick (Tokuyama-Japan), GO: Grandio (Voco-Germany), AB: Arabesque (Voco-Germany) and PS: Polofil Supra (Voco-Germany). The number of samples for each material was determined as 12 (n=12). Samples were prepared by using teflon molds, and cell viability rates were evaluated by MTT analysis as a result of contact of the GFBCs with the samples for 72 hours. Results: Cell viability rates of the groups were as follows; PS> AB> GO> ES> XF> GA. Conclusion: The structure of the material, the rate of the monomer, the type of monomer and the content of the filler are effective for the cytotoxicity of a material as a whole. The presence of triethylene glycol dimethacrylate and ethylene glycol dimethylacrylate monomers which increase the potential for toxicity of the material, and the contents of the filler (Fluroalumino silicate particles, iterium trifluoride particles, etc.) can affect the cytotoxicity.
Keywords: Composite resin; stem cell; cell viability; cytotoxicity; MTT assay
Objective: Restorative materials in contact with oral tissues may cause inflammatory, allergic, toxic, mutagenic and carcinogenic reactions in these tissues. Therefore, biocompatibility factor is a factor that must be kept in foreground in restorative materials. The aim of this study was to evaluate the cytotoxic effects of resin-containing filler materials on gingival fibroblast stem cells by 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) analysis. Material and Methods: Six different composite materials of the new generation (one bulk fill, one supra-nanohybrid, two nanohybrid, two micro-hybrid) were used in the study. The study groups were prepared as XF: X-tra Fill (Voco-Germany), GA: G-anial Posterior (GC Tokyo Japan), ES: Estelite Sigma Quick (Tokuyama-Japan), GO: Grandio (Voco-Germany), AB: Arabesque (Voco-Germany) and PS: Polofil Supra (Voco-Germany). The number of samples for each material was determined as 12 (n=12). Samples were prepared by using teflon molds, and cell viability rates were evaluated by MTT analysis as a result of contact of the GFBCs with the samples for 72 hours. Results: Cell viability rates of the groups were as follows; PS> AB> GO> ES> XF> GA. Conclusion: The structure of the material, the rate of the monomer, the type of monomer and the content of the filler are effective for the cytotoxicity of a material as a whole. The presence of triethylene glycol dimethacrylate and ethylene glycol dimethylacrylate monomers which increase the potential for toxicity of the material, and the contents of the filler (Fluroalumino silicate particles, iterium trifluoride particles, etc.) can affect the cytotoxicity.
Keywords: Composite resin; stem cell; cell viability; cytotoxicity; MTT assay
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