Turkiye Klinikleri Journal of Forensic Medicine and Forensic Sciences

.: ORIGINAL RESEARCH
Farklı Adli Biyolojik Örnekler Üzerinde Gelişen Mantar Türleri ile İlgili Bazı Doğal Ekstraktların Antifungal Etkileri: Deneysel Çalışmalar
Antifungal Effects of Some Natural Extracts on Fungal Species Belonging to Different Forensic Biological Samples: Experimental Studies
Hüseyin AVCIa, Şahlan ÖZTÜRKb, Belma ASLIMc
aNevşehir Hacı Bektaş Veli Üniversitesi Fen Bilimleri Enstitüsü, Çevre Mühendisliği ABD, Nevşehir, TÜRKİYE
bNevşehir Hacı Bektaş Veli Üniversitesi Mühendislik-Mimarlık Fakültesi, Çevre Mühendisliği Bölümü, Nevşehir, TÜRKİYE
cGazi Üniversitesi Fen Fakültesi, Biyoloji Bölümü, Ankara, TÜRKİYE
Turkiye Klinikleri J Foren Sci Leg Med. 2021;18(3):215-25
doi: 10.5336/forensic.2021-82250
Article Language: TR
Full Text
ÖZET
Amaç: Bu çalışmada, İstanbul Kriminal Polis Laboratuvarı Müdürlüğüne gönderilen biyolojik materyaller üzerinden delil bütünlüğüne herhangi bir zarar vermeyecek şekilde alınan örneklerden izole edilerek tanımlanan mantar izolatları üzerinde bazı bitkilere ait ekstraktlar uygulanarak, bu bitkilerin antifungal etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Farklı biyolojik örneklerden elde edilen izolatlar, ileri mikrobiyolojik tanı yöntemleri (API ve matriks aracılı lazer desorpsiyon/iyonizasyon-uçuş zamanı-kütle spektrometresi) ile tanımlanmıştır. Bu izolatların ticari bir antifungal olan Oceral'a karşı dirençlilik durumları, agar kuyucuk difüzyon metodu ile belirlenmiştir. Oceral'a direnç gösteren izolatlar üzerinde ülkemizde doğal olarak yetişen Cotinus coggygria, Tanacetum albipannosum, Lavandula stoechas, Juglans regia ve Hibiscus sabdariffa bitki ekstraktlarının, agar kuyucuk yöntemiyle antifungal etkileri çalışılmış ve en etkili bitki ekstraktları tespit edilmiştir. Bulgular: Tanımlama sonucuna göre 40 izolatın %82,5'i Cryptococcus laurentii, %10'u Candida guilliermondii, %5'i Candida albicans ve %2,5'i Candida tropicalis olarak tespit edilmiştir. Oceral'a karşı en çok direnç gösteren izolatların sırasıyla C. laurentii H-40, C. albicans H-27, C. laurentii H-39, C. laurentii H-9, C. laurentii H-16 ve C. guilliermondii H-32 olduğu saptanmıştır. C. coggygria, T. albipannosum ile L. stoechas bitki ekstraktlarının, Oceral'dan daha fazla etki gösterdiği tespit edilmiştir. Sonuç: Bu çalışma ile pütrifiye olmuş kanlı biyolojik materyaller üzerinden en fazla izole edilen tür C. laurentii olmuştur. Kullanılan bitki ekstraktlarının, Oceral'a nazaran yüksek derecede antifungal etki ettiği saptanmıştır. Bu çalışma ile ciddi yan etkilere sahip olan kimyasal antifungal ilaçlara alternatif olarak yan etkisi düşük, doğal bir ürünün kullanımı önerilmektedir.

Anahtar Kelimeler: Olay yeri; kriminalistik; Candida; Cryptococcus; antifungal etki
ABSTRACT
Objective: In this study, it was aimed to investigate the antifungal effects of some plants against to yeasts isolated from the samples taken from the biological materials sent to the İstanbul Criminal Police Laboratory Directorate without causing any damage to the integrity of the evidence. Material and Methods: Isolates obtained from different biological samples were identified by advanced microbiological identification methods (API and matrix-assisted laser desorption/ionization-time of flight mass spectrometry). Resistance of the isolates to Oceral, was determined by agar well diffusion method. The antifungal activity of plant extracts which naturally grown in our country such as Cotinus coggygria, Tanacetum albipannosum, Lavandula stoechas, Juglans regia and Hibiscus sabdariffa were examined on the resistant isolates by agar well diffusion method and the most effective plant extracts were detected. Results: According to identification 82.5% of isolates are Cryptococcus laurentii, 10% Candida guilliermondii, 5% Candida albicans and 2.5% Candida tropicalis. It was determined that isolates having more resistance to Oceral are C. laurentii H-40, C. albicans H-27, C. laurentii H-39, C. laurentii H-9, C. laurentii H-16 and C. guilliermondii H-32, respectively. It was determined that C. coggygria, T. albipannosum and L. stoechas plant extracts have higher antifungal activity on resistant isolates than Oceral. Conclusion: This study confirmed that most isolated species from putrified bloody biological materials is determined as C. laurentii. The plant extracts were found to have a higher antifungal effect compared to Oceral. This study suggests the use of a natural product with low side effects as an alternative to chemical antifungal drugs that have serious side effects.

Keywords: Crime scene; criminalistics; Candida; Cryptococcus; antifungal effect
REFERENCES:
  1. Breeze RG, Budowle B, Schutzer SE, eds. Anğ Ö, çeviri editörü. Adli Mikrobiyoloji. İstanbul: Nobel Tıp Kitapevleri; 2011. p.417.
  2. Polat O. Kriminoloji ve Kriminalistik Üzerine Notlar. 1. Baskı. Ankara: Seçkin Yayınevi; 2004.
  3. Deniz T. Olay Yeri İncelemesinde Delilden Sanığa Gitmenin İnsan Haklarının Korunmasındaki Önemi. İstanbul: Maltepe Üniversitesi; 2016. [Link] 
  4. Çetinbaş S, Kemeriz F, Göker G, Biçer İ, Velioğlu YS. İnsan mikrobiyomu: beslenme ve sağlik üzerindeki etkileri [Human microbiome: nutritional and health effects]. Akademik Gıda. 2017;15(4):409-15. [Crossref] 
  5. Çöl BG, Aksu H. Gıda işletmelerinde ortam havasının mikrobiyel yükü üzerine etkili faktörler ve hava örnekleme teknikleri [The effective factors on microbial load of air in food plant and air sampling techniques]. Journal of İstanbul Veterinary Sciences. 2007;2:24-47. [Link] 
  6. Güven K. Genel Mikrobiyoloji. 3. Baskı. Eskişehir: Anadolu Üniversitesi Web-Ofset Tesisleri; 2011. [Link] 
  7. Arslan A, Ozkan Kose C, Sig AK, Dogan E, Esim O, et al. Evaluation of a novel oxxiconazole nitrate formulation: The thermosensitive gel. Saudi Pharmaceutical Journal 26 (2018) 665-72. [Crossref]  [PubMed]  [PMC] 
  8. Karakoç E, Yazgı H, Aktaş AE, Uyanık MH. Çeşitli Candida türlerinin iki farklı triazole duyarlılıklarının mikrodilüsyon yöntemi ile araştırılması [The investigation of the susceptibility of various Candida species to two different triazoles by microdilution method]. The Eurasian Journal of Medicine. 2007;39:173-7. [Link] 
  9. Ferreira-Paim K, Andrade-Silva L, Mora DJ, Lages-Silva E, Pedrosa AL, da Silva PR, et al. Antifungal susceptibility, enzymatic activity, PCR-fingerprinting and ITS sequencing of environmental Cryptococcus laurentii isolates from Uberaba, Minas Gerais, Brazil. Mycopathologia. 2012;174(1):41-52. [Crossref]  [PubMed] 
  10. Cafarchia C, Romito D, Coccioli C, Camarda A, Otranto D. Phospholipase activity of yeasts from wild birds and possible implications for human disease. Med Mycol. 2008;46(5):429-34. [Crossref]  [PubMed] 
  11. Furman-Kuklińska K, Naumnik B, Myśliwiec M. Fungaemia due to Cryptococcus laurentii as a complication of immunosuppressive therapy--a case report. Adv Med Sci. 2009;54(1): 116-9. [Crossref]  [PubMed] 
  12. Dorum M. Klinik örneklerden izole edilen mantarlara karşı bazı doğal ürünlerin antifungal etkileri. Nevşehir: Nevşehir Hacı Bektaş Veli Üniversitesi; 2016. Erişim tarihi: 05/10/2021. Erişim linki: [Link] 
  13. Benabdelkader T, Zitouni A, Guitton Y, Jullien F, Maitre D, Casabianca H, et al. Essential oils from wild populations of Algerian Lavandula stoechas L.: composition, chemical variability, and in vitro biological properties. Chem Biodivers. 2011;8(5):937-53. [Crossref]  [PubMed] 
  14. Öztürk B, Konyalıoğlu S. İzmir yöresindeki yabani Lavandula stoechas L. subsp. stoechas taksonundan elde edilen uçucu yağın bileşimi, antibakteriyel, antifungal ve antioksidan kapasitesi [Essential oil composition, antibacterial antifungal and antioxidant capacity of wild Lavandula stoechas L. subsp. stoechas from İzmir]. Anadolu J of AARI. 2005;15(1):61-72. [Link] 
  15. Zuzarte M, Gonçalves MJ, Cavaleiro C, Cruz MT, Benzarti A, Marongiu B, et al. Antifungal and anti-inflammatory potential of Lavandula stoechas and Thymus herba-barona essential oils. Industrial Crops and Products. 2013; 44:97-103. [Crossref] 
  16. Matić S, Stanić S, Mihailović M, Bogojević D. Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential. Saudi J Biol Sci. 2016;23(4):452-61. [Crossref]  [PubMed]  [PMC] 
  17. Şen S, Hafızoğlu H. Bazı bitkisel ekstraktlarin toprakla temasta odun koruyucu etkinliklerinin belirlenmesi [Determination of wood preservative effects of some plant extracts contacting with soil]. Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi. 2008;4(1-2):69-82. [Link] 
  18. Marčetić M, Božić D, Milenković M, Male?ević N, Radulović S, Kovačević N. Antimicrobial, antioxidant and anti-inflammatory activity of young shoots of the smoke tree, Cotinus coggygria Scop. Phytother Res. 2013;27(11):16 58-63. [Crossref]  [PubMed] 
  19. Zhang F, Zhou J, Shi Y, Karaisz K. Identification of antioxidative ingredients from feverfew (Tanacetum parthenium) extract substantially free of parthenolide and other alpha unsaturated gamma-lactones. Open J Anal Bioanal Chem. 2019;3(1):076-082. [Crossref] 
  20. Kodak E, Erdoğan İ, Bani B, Acar Şahin A, Pınar NM. Leaf micromorphology of some Tanacetum L. (Asteraceae) taxa in Turkey. GU J Sci. 2017;30(4):30-41. [Link] 
  21. Arıtuluk ZC, Gençler Özkan AM, Ezer N. Comparative anatomical studies on three Tanacetum L. taxa (Asteraceae) growing in Turkey. Erzincan University Fen Bilimleri Enstitüsü Dergisi Journal of Science and Technology. 2019;12(1):336-48. [Link] 
  22. Joshan DS, Singh SK. Investigational study of Juglans regia extract and quercetin against photoaging. Biomedicine & Aging Pathology. 2013;3(4):193-200. [Crossref] 
  23. Yiğit D, Yiğit N, Aktaş E, Özgen U. Ceviz (Juglans regia l.)'in antimikrobiyal aktivitesi [Antimicrobial activity of walnut (Juglans regia L.)]. Türk Mikrobiyol Cem Der. 2009;39(1-2):7-11. [Link] 
  24. Gedik S. Çukurova koşullarında farklı ekim zamanlarının kerkede (Hibiscus sabdariffa L.) bitkisinin çanak yaprak verimi ve kalitesine etkisi. Adana: Çukurova Üniversitesi; 2014. (Erişim tarihi: 05/10/2021. Erişim linki: [Link] 
  25. Okasha MAM, Abubakar MS, Bako IG. Study of the effect of aqueous Hibiscus sabdariffa linn seed extract on serum prolactin level of lactating female albino rats. European Journal of Scientific Research. 2008;22(4):575-83. [Link] 

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