.: ORİJİNAL ARAŞTIRMA
Güncel Tam Seramik Sistemlerde Yüzey Topografisi ve Bakteri Adezyonunun İncelenmesi
Investigation of the Surface Topography and Bacterial Adhesion in Current All Ceramic Systems
Turkiye Klinikleri J Dental Sci. 2020;26(2):212-8
doi: 10.5336/dentalsci.2019-64780
Makale Dili: TR
Tam Metin
ÖZET
Amaç: Bu in vitro çalışmanın amacı; farklı tam seramik sistemlerde yüzey pürüzlülüğü ve Streptococcus mutans adezyonunun incelenmesidir. Gereç ve Yöntemler: Altı farklı CAD-CAM blok materyali [Vita Mark II (VMII), IPS e-max CAD (IPS), Vita Enamic (VE), Lava Ultimate (LU), Vita Suprinity (VS) ve Vita YZ ST (YZST)] kullanılarak 10,0 mm çapında ve 2,0±0,3 mm kalınlığında 54 adet silindir şekilli test örneği hazırlandı. Örneklerin yüzey pürüzlülüğü profilometre cihazı ile ölçüldü. Ardından steril edilen test örnekleri, pelikıl tabakası oluşturmak için müsin içeren yapay tükürük ile kaplandı ve 24 saatlik S. mutans adezyonu test edildi. Elde edilen veriler, tek yönlü varyans analizi (ANOVA) ve Tukey HSD post-hoc testleri ile istatistiksel olarak karşılaştırıldı. İstatistiksel anlamlılık düzeyi p<,05 olarak belirlendi. Bulgular: En yüksek ortalama yüzey pürüzlülüğü değeri YZ-ST grubunda (2.,07±0,71 μm), en düşük ortalama yüzey pürüzlülüğü değeri ise LU grubunda (0,39±0,15 μm) tespit edildi. Farklı tam seramik sistemlerin yüzey pürüzlülüğü bulgularına göre çoktan aza doğru sıralaması; YZ-ST> VMII> IPS> VS> VE> LU şeklindedir. En yüksek bakteri adezyonu ortalama değeri YZ-ST grubunda, en düşük ortalama bakteri adezyonu değeri ise VE grubunda tespit edildi. VE, LU ve IPS grupları arasında yapılan karşılaştırmada, benzer bakteri adezyonu değerleri belirlendi (p>,05). Sonuç: Kullanılan tam seramik restoratif materyal seçeneği, restorasyonların yüzey pürüzlülüğü ve bakteri adezyonu değerlerini etkilemektedir.
Anahtar Kelimeler: Tam seramik sistemler; yüzey pürüzlülüğü; bakteri adezyonu
Amaç: Bu in vitro çalışmanın amacı; farklı tam seramik sistemlerde yüzey pürüzlülüğü ve Streptococcus mutans adezyonunun incelenmesidir. Gereç ve Yöntemler: Altı farklı CAD-CAM blok materyali [Vita Mark II (VMII), IPS e-max CAD (IPS), Vita Enamic (VE), Lava Ultimate (LU), Vita Suprinity (VS) ve Vita YZ ST (YZST)] kullanılarak 10,0 mm çapında ve 2,0±0,3 mm kalınlığında 54 adet silindir şekilli test örneği hazırlandı. Örneklerin yüzey pürüzlülüğü profilometre cihazı ile ölçüldü. Ardından steril edilen test örnekleri, pelikıl tabakası oluşturmak için müsin içeren yapay tükürük ile kaplandı ve 24 saatlik S. mutans adezyonu test edildi. Elde edilen veriler, tek yönlü varyans analizi (ANOVA) ve Tukey HSD post-hoc testleri ile istatistiksel olarak karşılaştırıldı. İstatistiksel anlamlılık düzeyi p<,05 olarak belirlendi. Bulgular: En yüksek ortalama yüzey pürüzlülüğü değeri YZ-ST grubunda (2.,07±0,71 μm), en düşük ortalama yüzey pürüzlülüğü değeri ise LU grubunda (0,39±0,15 μm) tespit edildi. Farklı tam seramik sistemlerin yüzey pürüzlülüğü bulgularına göre çoktan aza doğru sıralaması; YZ-ST> VMII> IPS> VS> VE> LU şeklindedir. En yüksek bakteri adezyonu ortalama değeri YZ-ST grubunda, en düşük ortalama bakteri adezyonu değeri ise VE grubunda tespit edildi. VE, LU ve IPS grupları arasında yapılan karşılaştırmada, benzer bakteri adezyonu değerleri belirlendi (p>,05). Sonuç: Kullanılan tam seramik restoratif materyal seçeneği, restorasyonların yüzey pürüzlülüğü ve bakteri adezyonu değerlerini etkilemektedir.
Anahtar Kelimeler: Tam seramik sistemler; yüzey pürüzlülüğü; bakteri adezyonu
ABSTRACT
Objective: The purpose of this in vitro study was to evaluate the surface roughness and Streptococcus mutans adhesion in different all ceramic systems. Material and Methods: A total of 54 cylindrical test specimens were prepared using 6 different CAD-CAM blocks [Vita Mark II (VMII), IPS e-max CAD (IPS), Vita Enamic (VE), Lava Ultimate (LU), Vita Suprinity (VS) and Vita YZ ST (YZ-ST)] with a diameter of 10.0 mm and a thickness of 2.0±0.3 mm. The surface roughness of the specimens was measured by a profilometer. Subsequently, the sterilized specimens were coated with mucin-containing artificial saliva to form the pellicle layer, and then the 24-hour S. Mutans adhesion was tested. The obtained data were statistically compared using one-way analysis of variance (ANOVA) and Tukey HSD posthoc tests. Statistical significance was set at p<.05. Results: The highest mean surface roughness was obtained in the YZ-ST group (2.07±0.71 μm) while the lowest surface roughness was found in the LU group (0.39±0.15 μm). The surface roughness findings of different all ceramic systems was as YZ-ST> VMII> IPS> VS> VE> LU. The highest bacterial adhesion was found in the YZ-ST group and the lowest bacterial adhesion was seen in the VE group. Similar bacterial adhesion values were determined between VE, LU and IPS groups (p>.05). Conclusion: The selected all ceramic restorative material option may affect the surface roughness and bacterial adhesion values of the restorations.
Keywords: All ceramic systems; surface roughness; bacterial adhesion
Objective: The purpose of this in vitro study was to evaluate the surface roughness and Streptococcus mutans adhesion in different all ceramic systems. Material and Methods: A total of 54 cylindrical test specimens were prepared using 6 different CAD-CAM blocks [Vita Mark II (VMII), IPS e-max CAD (IPS), Vita Enamic (VE), Lava Ultimate (LU), Vita Suprinity (VS) and Vita YZ ST (YZ-ST)] with a diameter of 10.0 mm and a thickness of 2.0±0.3 mm. The surface roughness of the specimens was measured by a profilometer. Subsequently, the sterilized specimens were coated with mucin-containing artificial saliva to form the pellicle layer, and then the 24-hour S. Mutans adhesion was tested. The obtained data were statistically compared using one-way analysis of variance (ANOVA) and Tukey HSD posthoc tests. Statistical significance was set at p<.05. Results: The highest mean surface roughness was obtained in the YZ-ST group (2.07±0.71 μm) while the lowest surface roughness was found in the LU group (0.39±0.15 μm). The surface roughness findings of different all ceramic systems was as YZ-ST> VMII> IPS> VS> VE> LU. The highest bacterial adhesion was found in the YZ-ST group and the lowest bacterial adhesion was seen in the VE group. Similar bacterial adhesion values were determined between VE, LU and IPS groups (p>.05). Conclusion: The selected all ceramic restorative material option may affect the surface roughness and bacterial adhesion values of the restorations.
Keywords: All ceramic systems; surface roughness; bacterial adhesion
REFERANSLAR:
- Kelly JR, Benetti P. Ceramic materials in dentistry: historical evolution and current practice. Aust Dent J. 2011;56 Suppl 1:84-96. [Crossref] [PubMed]
- Fasbinder DJ. Digital dentistry: innovation for restorative treatment. Compend Contin Educ Dent. 2010;31 Spec No 4:2-11.
- Nguyen JF, Ruse D, Phan AC, Sadoun MJ. High-temperature-pressure polymerized resin infiltrated ceramic networks. J Dent Res. 2014;93(1):62-7. [Crossref] [PubMed] [PMC]
- Conrad HJ, Seong WJ, Pesun IJ. Current ceramic materials and systems with clinical recommendations: a systematic review. J Prosthet Dent. 2007;98(5):389-404. [Crossref]
- Rusin RP, Häberlein I, Schmid B, Stöger H, Hauke M, Brown AM. Plaque growth and activity on a resin nanoceramic CAD/CAM material. Dent Mater. 2013;29(1):24-5. [Crossref]
- Flemming HC, Wingender J. The biofilm matrix. Nat Rev Microbiol. 2010;8(9):623-33. [Crossref] [PubMed]
- Bayrak GD, Sandalli N, Selvi-Kuvvetli S, Topcuoglu N, Kulekci G. Effect of two different polishing systems on fluoride release, surface roughness and bacterial adhesion of newly developed restorative materials. J Esthet Restor Dent. 2017;29(6):424-34. [Crossref] [PubMed]
- Cazzaniga G, Ottobelli M, Ionescu A, Garcia-Godoy F, Brambilla E. Surface properties of resin-based composite materials and biofilm formation: a review of the currentliterature. Am J Dent. 2015;28(6):311-20.
- Bremer F, Grade S, Kohorst P, Stiesch M. In vivo biofilm formation on different dental ceramics. Quintessence Int. 2011;42(7):565-74.
- Lemos JA, Quivey RG Jr, Koo H, Abranches J. Streptococcus mutans: a new Gram-positive paradigm? Microbiology. 2013;159(Pt 3):436-45. [Crossref] [PubMed] [PMC]
- Hahnel S, Rosentritt M, Handel G, Bürgers R. Surface characterization of dental ceramics and initial Streptococcal adhesion in vitro. Dent Mater. 2009;25(8):969-75. [Crossref] [PubMed]
- Kawai K, Urano M, Ebisu S. Effect of surface roughness of porcelain on adhesion of bacteria and their synthesizing glucans. J Prosthet Dent. 2000;83(2):664-7. [Crossref]
- Ionescu A, Wutscher E, Brambilla E, Schneider-Feyrer S, Giessibl F, Hahnel S. Influence of surface properties of resin-based composites on in vitro Streptococcus mutans biofilm development. Eur J Oral Sci. 2012;120(5): 458-65. [Crossref] [PubMed]
- Kakaboura A, Fragouli M, Rahiotis C, Silikas N. Evaluation of surface characteristics of dental composites using profilometry, scanning electron, atomic force microscopy and gloss-meter. J Mater Sci Mater Med. 2007;18(1): 155-63. [Crossref] [PubMed]
- Whitehead SA, Shearer AC, Watts DC, Wilson NH. Comparison of methods for measuring surface roughness of ceramic. J Oral Rehabil. 1995;22(6):421-7. [Crossref] [PubMed]
- Zitzmann NU, Berglundh T. Definition and prevalence of peri-im¬plant diseases. J Clin Periodontol. 2008;35(8 Suppl):286-91. [Crossref] [PubMed]
- Koizumi H, Saiki O, Nogawa H, Hiraba H, Okazaki T, Matsumura H. Surface roughness and gloss of current CAD/CAM resin composites before and after toothbrush abrasion. Dent Mater J. 2015;34(6):881-7. [Crossref] [PubMed]
- Mörmann WH, Stawarczyk B, Ender A, Sener B, Attin T, Mehl A. Wear characteristics of current aesthetic dental restorative CAD/CAM materials: two-body wear, gloss retention, roughness and Martens hardness. J Mech Behav Biomed Mater. 2013;20(1):113-25. [Crossref] [PubMed]
- Stawarczyk B, Frevert K, Ender A, Roos M, Sener B, Wimmer T. Comparison of four monolithic zirconia materials with conventional ones: contrast ratio, grain size, four-point flexural strength and two-body wear. J Mech Behav Biomed Mater. 2016;59(6):128-38. [Crossref] [PubMed]
- Şen N, Tuncelli B, Göller G. Surface deterioration of monolithic CAD/CAM restorative materials after artificial abrasive toothbrushing. J Adv Prosthodont. 2018;10(4):271-8. [Crossref] [PubMed] [PMC]
- Yuan C, Wang X, Gao X, Chen F, Liang X, Li D. Effects of surface properties of polymer-based restorative materials on early adhesion of Streptococcus mutans in vitro. J Dent. 2016;54(1):33-40. [Crossref] [PubMed]
- Meier R, Hauser-Gerspach I, Lüthy H, Meyer J. Adhesion of oral streptococci to all-ceramics dental restorative materials in vitro. J Mater Sci Mater Med. 2008;19(3):3249-53. [Crossref] [PubMed]
- van Loosdrecht MCM, Lyklema J, Norde W, Schraa G, Zehnder AJB. Electrophoretic mobility and hydrophobicity as a measure to predict the initial steps of bacterial adhesion. Appl Environ Microbiol. 1987;53(2):1898-901. [Crossref] [PubMed] [PMC]
- Lampin M, Warocquier-Clérout R, Legris C, Degrange M, Sigot-Luizard MF. Correlation between substratum surface roughness and wettability, cell adhesion, and cell migration. J Biomed Mater Res. 1997;36(1):99-108. [Crossref]
- Zarone F, Russo S, Sorrentino R. From porcelain-fused-to-metal to zirconia: clinical and experimental considerations. Dent Mater. 2011;27(1):83-96. [Crossref] [PubMed]
- Milleding P, Gerdes S, Holmberg K, Karlsson S. Surface energy of non-corroded and corroded dental ceramic materials before and after contact with salivary proteins. Eur J Oral Sci. 1999;107(11):384-92. [Crossref] [PubMed]
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