Journal of Traditional Medical Complementary Therapies

.: ORIGINAL RESEARCH
An Experimental Study on the Effect of Platelet-Rich Plasma Application on Skin Graft Healing
Trombositten Zengin Plazma Uygulamasının Deri Grefti İyileşmesi Üzerine Etkisinin Deneysel Olarak Değerlendirilmesi
Niyazi GÜLSÜN a, Mehmet Fatih AKKOÇb, Emin KAPIc, Caferi Tayyar SELÇUK b, Mehmet BOZKURTd, Veysi AKPOLAT e, Yusuf ÇELİKf, Ulaş ALABALIKg, İsmail YILDIZh
aClinic of Plastic, Reconstructive and Aesthetic Surgery, Gazi Yaşargil Training and Research Hospital, Diyarbakır, Türkiye
bDepartment of Plastic, Reconstructive and Aesthetic Surgery, Dicle University Faculty of Medicine, Diyarbakır, Türkiye
cDepartment of Plastic, Reconstructive and Aesthetic Surgery, University of Health Sciences Adana Faculty of Medicine, Adana, Türkiye
dDepartment of Plastic, Reconstructive and Aesthetic Surgery, University of Health Sciences Bağcılar Research and Training Hospital, İstanbul, Türkiye
eDepartment of Biophysics, Dicle University Faculty of Medicine, Diyarbakır, Türkiye
fDepartment of Biostatistics, Biruni University Faculty of Medicine, İstanbul, Türkiye
gDepartment of Pathology, Dicle University Faculty of Medicine, Diyarbakır, Türkiye
hDepartment of Biostatistics, Dicle University Faculty of Medicine, Diyarbakır, Türkiye
J Tradit Complem Med. 2022;5(1):40-9
doi: 10.5336/jtracom.2021-85553
Article Language: EN
Full Text
ABSTRACT
 Objective: There is new information increasingly added about wound healing, which is one of the most complex physiological processes. In this study, it is aimed to evaluate the effect of plateletrich plasma on the wound healing process in pursuit of creation of skin defect on the back skin of the rat and application of graft to it. Material and Methods: The study was planned on 30 randomly selected inbred Sprague-Dawley rats. In the rats in the experimental group, 3x2 cm full-thickness skin defect was created after the back area was shaved. The skin taken from the defected area was thinned and turned into a full-thickness skin graft and sutured again to the area where it was taken. Platelet-rich plasma was applied under the graft to the rats in the 1st group, and physiological saline was applied to the 2nd group. The 3rd group did not undergo any surgical procedure, and it was created as the control group. Results: Significant differences were observed in glucose, lactate, glycerol, and pyruvate values in the tissue in Group 1, compared to other groups. In the histopathological evaluation, a significant decrease was detected in the rate of inflammation and edema in the Group 1, in comparison with the other groups. Conclusion: Based on the data obtained from this study, it was taken into consideration by us that platelet-rich plasma application is a method that can be applied in addition to the classical wound care, especially in the cases with wound healing problems.

Keywords: Wound healing; platelet-rich plasma; microdialysis; skin grafting; angiogenesis
ÖZET
 Amaç: Oldukça karmaşık fizyolojik süreçlerden biri olan yara iyileşmesi ile ilgili hâlen yeni bilgilere ulaşılmaktadır. Bu çalışmada, sıçan sırt derisinde deri defekti oluşturulması ve sonrasında greft uygulamasını takiben trombositten zengin plazmanın yara iyileşme sürecine etkisinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: Çalışma, randomize olarak seçilen 30 adet inbred Sprague-Dawley sıçan üzerinde planlandı. Deney grubundaki sıçanlarda, anestezi sonrası sırt bölgesi tıraşlandıktan sonra 3x2 cm boyutlu tam kat deri defekti oluşturuldu. Defekt oluşturulan alandan alınan deri inceltilerek tam kalınlıkta deri grefti hâline getirildi ve alınan yere tekrar sütüre edildi. Birinci gruptaki sıçanlara greft altına trombositten zengin plazma, 2. gruba ise serum fizyolojik uygulandı. Üçüncü gruba ise herhangi bir cerrahi işlem uygulanmadı ve kontrol grup olarak oluşturuldu. Bulgular: Mikrodiyaliz verileri karşılaştırıldığında Grup 1'de, diğer gruplara kıyasla dokudaki glukoz, laktat, gliserol ve pirüvat değerlerinde anlamlı farklar saptandı. Histopatolojik degerlendirmede ise Grup 1'de, di ̆ ğer gruplara kıyasla inflamasyon ve ödem oranında belirgin azalma saptandı. Sonuç: Bu çalışmadan elde edilen verilere dayanarak, trombositten zengin plazma uygulamasının, özellikle yara iyileşme problemi bulunan durumlarda, klasik yara bakımına ek olarak uygulanabilecek bir yöntem olduğunu düşünmekteyiz.

Anahtar Kelimeler: Yara iyileşmesi; trombositten zengin plazma; mikrodiyaliz; deri grefti; anjiyogenezis
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