Turkiye Klinikleri Journal of Veterinary Sciences

.: ORIGINAL RESEARCH
Circulating Serum Zonulin Levels Before and After Probiotic Enema Treatment in Dogs with Atopic Dermatitis: Randomized Clinical Study
Atopik Dermatitli Köpeklerde Probiyotik Enema Sağaltımı Öncesi ve Sonrası Dolaşım Serum Zonulin Seviyesi: Randomize Klinik Çalışma
Kerem URALa, Hasan ERDOĞANa, Songül ERDOĞANa, İlker CAMKERTENb, Nurcan ŞAHİNa
aDepartment of Internal Medicine, Aydın Adnan Menderes University Faculty of Veterinary, Aydın, TURKEY
bDepartment of Internal Medicine, Aksaray University Faculty of Veterinary, Aksaray, TURKEY
Turkiye Klinikleri J Vet Sci. 2021;12(2):70-8
doi: 10.5336/vetsci.2021-85829
Article Language: EN
Full Text
ABSTRACT
 Objective: In the present study, we investigated the benefits of rectal enema probiotic combination in dogs with atopic dermatitis and detected circulating serum zonulin levels in these. Material and Methods: An open-label, self-controlled case series involved 6 privately owned dogs with atopic dermatitis. Diagnosis involved corneometric analytes, Canine Atopic Dermatitis Extent and Severity Index version 4 (CADESI-04) scores, visual analogue scale (VAS) pruritus scores and in vitro allergen specific tests. Faecal samples were forwarded to dual indexing one-step polymerase chain reaction and 16S rRNA targeted metagenomics in an attempt to determine gut microbiota alterations before and after rectal enema probiotic once daily for 10 days. Results: Following rectal enema probiotic treatment, as the in the vast majority Prevotella genus was significantly detected in association with clinical cure and recovery. Median CADESI-04 (p=0.028) and VAS pruritus scores (p=0.028) along with circulating zonulin (p=0.026) levels were decreased in atopic dermatitis cases on day 10 in contrast to 0th day values. Corneometric analysis involving median epidermal pH (p=0.027) and hydration values (p=0.028) revealed a significant improvement. Conclusion: This positive impact on skin inflammation and diminshed pruritus after application of rectal enema probiotic in this model of atopic dermatitis in dogs probably presented withdrawal of chronic barrier disruption and healed tigh junctions. The selected Lactobacillus probiotic strains used as an enema potentially regulate on skin health by the manipulation of the gut microbiome resulted with intestinal balance.

Keywords: Canine atopy; intestinal microbiome; probiotics; rectal enema; zonulin
ÖZET
 Amaç: Bu çalışmada, atopik dermatitisli köpeklerde probiyotik kombinasyonlarının rektal uygulama yararlarının araştırılması ve bunlarda serum zonulin seviyelerinin belirlenmesi amaçlandı. Gereç ve Yöntemler: Açık-uçlu, öz deneyimli olgu serisi atopik dermatitisli 6 sahipli köpekten oluştu. Tanıda korneometrik analiz, Köpeklerde Atopik Dermatit Yaygınlık ve Şiddet İndeksi-04 [Canine Atopic Dermatitis Extent and Severity Index version 4 (CADESI-04)] skoru, vizüel analog skala (VAS) kaşıntı skoru ve in vitro alerjen spesifik test kullanıldı. Dışkı örnekleri, 10 gün boyunca günde 1 kez rektal enema probiyotik öncesi ve sonrasında bağırsak mikrobiyota değişikliklerini belirlemek amacıyla metagenomikleri hedefleyen ikili indeksleme tek adımlı polimeraz zincir reaksiyonu ve 16S rRNA analizine gönderildi. Bulgular: Rektal enema probiyotik sağaltımını takiben klinik iyileşme ve tedaviyle ilişkili büyük çoğunluğu Prevotella cinsi olacak şekilde önemli bulundu. Ortalama CADESI-04 (p=0,028) ve VAS kaşıntı skoru (p=0,028) ile zonulin (p=0,026) seviyesi 0. gün değerlerine kıyasla 10. günde azaldı. Korneometrik analizlerden epidermal pH (p=0,027) ve hidrasyon değeri (p=0,028) belirgin iyileşme gösterdi. Sonuç: Köpeklerde, bu atopik dermatitis modelinde rektal enema probiyotik uygulanmasından sonra deri yangısı ve azalan kaşıntı üzerindeki olumlu etki, muhtemelen kronik bariyer bozulmasının gerilemesi ve sıkı bağlantıların iyileşmesini temsil etmektedir. Enema kullanımında tercih edilen Lactobacillus probiyotik suşları, intestinal denge ile sonuçlanan bağırsak mikrobiyotasının manipüle edilmesi ile deri sağlığını potansiyel olarak düzenledi.

Anahtar Kelimeler: Kanin atopi; intestinal mikrobiyom; probiyotikler; rektal enema; zonulin
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