Turkiye Klinikleri Journal of Medical Sciences

.: ORIGINAL RESEARCH
Investigation of HPV Infection and P53 Expression in Laryngeal Verrucous Carcinoma
Larengeal Verrüköz Karsinomlarda HPV Enfeksiyonu ve P53 Ekspresyonu
Mehmet Ali TUNÇBİLEKa, Çağlar ÇALLIa, Semih ÖNCELa, Ercan PINARa, Aylin ÖRGEN ÇALLI MDb
Departments of aHead and Neck Surgery, bPathology, İzmir Atatürk Training and Research Hospital, İzmir
Turkiye Klinikleri J Med Sci. 2010;30(1):194-200
doi: 10.5336/medsci.2008-8340
Article Language: EN
Full Text
ABSTRACT
 Objective: Verrucous carcinoma is an uncommon, well-differentiated variant of squamous cell carcinoma that is regarded as a low grade, locally invasive malignant tumor with rare potential for distant metastasis. Although the pathogenesis of verrucous carcinoma is unknown, many possible causes have been proposed. The leading theories include human papillomavirus (HPV) infection and chemical carcinogenesis. The aim of this study is to analyse the status of the p53 gene and human papillomavirus (HPV) infection in verrucous carcinoma of larynx, and to investigate the correlation of p53 expression and HPV infection in verrucous carcinoma of the larynx. Material and Methods: A retrospective study has been performed between the years of 1994 and 2004, comprising 21 consecutive patients with verrucous carcinoma of larynx. Detailed clinical parameters have also been reviewed. HPV identification was accomplished with polymerase chain reaction. p53 immunohistochemistry was performed on 21 verrucous carcinoma using polyclonal serum CM-1. Results: The HPV infection has been detected in in all of the twenty-one cases of verrucous carcinomas. p53 immunohistochemistry was performed on 21 HPV containing verrucous carcinomas and showed the expression of p53 in 14 cases. No statistically significant differences were found between p53 expression and stage. Conclusion: The data indicate that HPV and the increased expression of p53 can coexist in verrucous carcinomas. Our study suggests that verrucous carcinomas of the larynx may arise through a still unknown mechanism which causes p53 inactivation or HPV infection.We think that p53 overexpression and oncogenic HPV infection may cooperate in the establishment of malignant cells in verrucous carcinoma of the larynx. The interaction between the HPV infection and certain cellular oncogenes such as p53 needs further studies.

Keywords: Carcinoma, verrucous; larynx; p53 protein, human; papillomavirus infections
ÖZET
 Amaç: Nadir metastaz potansiyeli bulunan düşük dereceli lokal invaziv malign tümör olarak bilinen verrüköz karsinom, skuamöz hücreli karsinomun iyi diferansiye bir varyantıdır. Verrüköz karsinomun patogenezi bilinmese de bazı olası nedenler öne sürülmüştür. Bunların başında human papilloma virus enfeksiyonu ve kimyasal karsinogenez gelmektedir. Çalışmamızın amacı larinksin verrüköz karsinomlarında p53 gen ve human papilloma virus enfeksiyonunun analizi ve p53 ekspresyonu ile human papilloma virus arasındaki ilişkinin değerlendirilmesidir. Gereç ve Yöntemler: Retrospektif çalışma 1994-2004 yılları arasında tanı almış 21 larengeal verrüköz karsinomlu olguda yapılmıştır. Detaylı klinik parametreler gözden geçirilmiştir. Human papilloma virus identifikasyonu polimeraz zincir reaksiyonu ile yapılmıştır. P53 immun boyası, 21 larengeal verrüköz karsinomlu olguda polyclonal CM-1 serumu kullanılarak uygulanmıştır. Bulgular: Human papilloma virus enfeksiyonu 21 larengeal verrüköz karsinomlu olgunun 21inde (%100) saptanmıştır. Artmış p53 expresyonu ise 21 larengeal verrüköz karsinomlu olgunun 14ünde tespit edilmiştir. P53 ekspresyonu ile evre arasında istatistiksel olarak anlamlı bir ilişki saptanmamıştır. Sonuç: Çalışmamızda HPV enfeksiyonunun ve artmış p53 ekspresyonunun verrüköz karsinomlarda birarada bulunabileceği gösterilmiştir. Çalışmamız larengeal verrüköz karsinomunun p53 inaktivasyonuna veya human papilloma virus enfeksiyonuna neden olan henüz bilinmeyen bir mekanizma sonucu geliştiğini destekler niteliktedir. Sonuç olarak; elde ettiğimiz veriler human papilloma virus enfeksiyonu ile p53 overekspresyonunun larengeal verrüköz karsinomlu olgularda malign hücre gelişiminde birlikte hareket ettiğini düşündürmektedir. Human papilloma virus enfeksiyonu ile p53 gibi belli başlı onkogenler arasındaki ilişkinin ortaya konması için daha fazla sayıda çalışmaya ihtiyaç vardır.

Anahtar Kelimeler: Verrüköz karsinom; larenks; insan p53 proteini; papilloma virus enfeksiyonu

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