Journal of Traditional Medical Complementary Therapies

.: ORIGINAL RESEARCH
The Effect of Curcumin on Some Plasma Cytokine Levels in Experimentally-Induced Diabetic Rats
Diyabet Oluşturulan Ratlarda Kurkuminin Bazı Plazma Sitokin Düzeyleri Üzerine Etkisi
Ülkü SAYGILIa, Nurcan DÖNMEZb
aDepartment of Health Care Services, Selçuk University Vocational School of Health Services, Konya, TURKEY
bDepartment of Physiology, Selçuk University Faculty of Veterinary Medicine, Konya, TURKEY
J Tradit Complem Med. 2021;4(1):57-64
doi: 10.5336/jtracom.2020-79086
Article Language: EN
Full Text
ABSTRACT
Objective: The aim of this study was to determine the effect of curcumin on some plasma cytokines and insulin levels in diabetes mellitus, which has negative effects on many acute and chronic systems in humans and animals. Material and Methods: In the study, thirty adult male Wistar albino rats with similar live weights were used. The subjects used in the study were divided into four groups including Control (K), Diabetes (D), Curcumin (C), and Diabetes+Curcumin (DC). At the end of the experiment, interleukin-6 (IL-6), IL-10, insulin, tumor necrosis factor alpha (TNF-α), and C-reactive protein (CRP) levels in the blood samples taken from the subjects in the groups were determined. Results: IL-6 level was determined to increase significantly in experimentally induced diabetic rats compared to the other three groups (p<0.05). The IL-6 level in the C group was found to be similar to that in the K group whereas it was observed that the IL-6 level was significantly lower in the DC group, in which C was supplemented for the experimentally induced diabetic rats, than in the D group, and approached to the levels obtained in the K and C groups (p<0.05). The CRP level was significantly higher in the D group compared to the other three groups whereas the insulin level was prominently lower (p<0.05). In this study, it was observed that there was a positive and strong relationship between IL-6 and TNF and CRP, and a moderately strong and negative relationship with insulin Conclusion: In conclusion, it was seen that curcumin application does not cause a negative impact on healthy rats and that it is beneficial in reducing the negative effects of diabetes in streptozotocin-induced diabetic rats.

Keywords: Diabetes mellitus; cytokine; curcumin; insulin; streptozotocin
ÖZET
Amaç: Bu çalışmanın amacı; insan ve hayvanlarda akut ve kronik çok sayıda sistem üzerine olumsuz etkileri olan diabetes ellitusta, kurkumin verilmesinin bazı plazma sitokinleri ve insülin düzeyine etkisinin belirlemektir. Gereç ve Yöntemler: Çalışmada, canlı ağırlıkları birbirine yakın 30 adet yetişkin erkek Wistar Albino sıçan kullanıldı. Denemede kullanılan denekler Kontrol (K), Diyabet (D), Kurkumin (C) ve Diyabet+Kurkumin (DC) olmak üzere 4 gruba ayrıldı. Araştırmada, deneme sonunda gruplardaki deneklerden alınan kan örneklerinde interlökin-6 (IL-6), IL-10, insülin, tümör nekroz faktör alfa (TNF-α), C-reaktif protein (CRP) düzeyleri belirlendi. Bulgular: IL-6 düzeyinin diyabet oluşturulan sıçanlarda diğer 3 gruba göre önemli (p<0,05) oranda arttığı belirlendi. Kurkumin uygulanan grupta belirlenen IL-6 düzeyi kontrole benzerken, diyabet oluşturulduktan sonra C takviyesi yapılan DC grubunda ise IL-6 düzeyinin D grubuna göre önemli oranda düşük olduğu, K ve C gruplarından elde edilen düzeylere ise yaklaştığı gözlendi (p<0,05). IL-10 düzeyinin C grubunda D grubuna kıyasla daha yüksek olduğu, D grubuna kurkumin takviyesinden sonra DC grubunda da IL-10 seviyesinin önemli (p<0,05) oranda arttığı belirlendi. D grubunda CRP seviyesi diğer 3 gruba göre anlamlı bir şekilde yüksek iken insülin düzeyi ise belirgin bir şekilde düşük olarak belirlendi (p<0,05). Bu çalışmada, IL-6 ile TNF ve CRP arasında pozitif ve güçlü bir ilişki olduğu, insülin ile orta derecede güçlü ve negatif bir ilişki olduğu görülmüştür. Sonuç: Streptozotosin ile deneysel diyabet oluşturulan sıçanlarda kurkumin uygulaması sağlıklı sıçanlarda olumsuz bir etki oluşturmamasının yanı sıra diyabette de meydana gelen olumsuz etkileri hafifletmesi bakımından yararlı olduğu görülmektedir.

Anahtar Kelimeler: Diabetes mellitus; sitokin; kurkumin; insülin; streptozotosin
REFERENCES:
  1. Mancia SR, Trujillo J, Chaverri JP. Utility of curcumin for the treatment of diabetes mellitus: Evidence from preclinical and clinical studies. Journal of Nutrition & Intermediary Metabolism 2018;14:29-41. [Link] 
  2. Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract. 2010;87(1):4-14. [Crossref] [PubMed] 
  3. Cruz NG, Sousa LP, Sousa MO, Pietrani NT, Fernandes AP, Gomes KB. The linkage between inflammation and Type 2 diabetes mellitus. Diabetes Res Clin Pract. 2013;99(2):85-92. [Crossref] [PubMed] 
  4. Liu T, Yang W, Pang S, Yu S, Yan B. Functional genetic variants within the SIRT2 gene promoter in type 2 diabetes mellitus. Diabetes Res Clin Pract. 2018;137:200-7. [Crossref] [PubMed] 
  5. Conlon KC, Miljkovic MD, Waldmann TA. Cytokines in the Treatment of Cancer. J Interferon Cytokine Res. 2019;39(1):6-21. [Crossref] [PubMed] [PMC] 
  6. Li J, Liu B, Cai M, Lin X, Lou S. Glucose metabolic alterations in hippocampus of diabetes mellitus rats and the regulation of aerobic exercise. Behav Brain Res. 2019;364:447-56. [Crossref] [PubMed] 
  7. Yalçınkaya Z, Turan H, Demir H. [Relationship with cytokine of patients diabetes]. Uluslararası Bilimsel Araştırmalar Dergisi. 2017;449-51. [Link] 
  8. Emral R. [Adiponectin and other cytokines: medical education]. Turkey Clinics Journal of Medical Sciences. 2006;26(4):409-20. [Link] 
  9. Yarım GF, Kazak F. [Cytokine response in metabolic syndrome and its components]. Journal of Harran University Faculty of Veterinary Medicine 2016;5(1):90-9. [Link] 
  10. Yalçın AS, Yılmaz AM, Altundağ EM, Koçtürk S. [Anti-cancer effects of curcumin, quercetin and tea catechins]. Marmara Pharmaceutical Journal. 2016;21:19-29. [Crossref] 
  11. Belviranli M, Okudan N, Nurullahoğlu Atalık KE. Effects of curcumin supplementation on oxidant / antioxidant status of heart tissue in elderly rats. Journal of General Medicine. 2012;22(2):61-6. [Link] 
  12. Ghosh S, Banerjee S, Sil PC. The beneficial role of curcumin on inflammation, diabetes and neurodegenerative disease: A recent update. Food Chem Toxicol. 2015;83:111-24. [Crossref] [PubMed] 
  13. Maithilikarpagaselvi N, Sridhar MG, Swaminathan RP, Zachariah B. Curcumin prevents inflammatory response, oxidative stress and insulin resistance in high fructose fed male Wistar rats: Potential role of serine kinases. Chem Biol Interact. 2016;244:187-94. [Crossref] [PubMed] 
  14. Faydaoğlu E, Sürücüoğlu M. [History of the use of medical and aromatic plants and their economic importance]. Journal of Faculty of Forestry. 2011;11(1):52-67. [Link] 
  15. Shehzad A, Ha T, Subhan F, Lee YS. New mechanisms and the anti-inflammatory role of curcumin in obesity and obesity-related metabolic diseases. Eur J Nutr. 2011;50(3):151-61. [Crossref] [PubMed] 
  16. Senatorski G, Paczek L, Kropiewnicka E, Bartłomiejczyk I. [Cytokines in noninvasive diagnostics of diabetic nephropathy progression]. Pol Merkur Lekarski. 2002;13 Suppl 1:28-32. Polish. [PubMed] 
  17. Demir EA, Öz M, Alp Mİ, Gergerlioğlu HS. Levels of IL-6 and TNF-α in diabetic rats: effect of quercetin. Ibn Sina Journal of Medical Sciences. 2015;1(2):27-31.
  18. Dalla Vestra M, Mussap M, Gallina P, Bruseghin M, Cernigoi AM, Saller A, et al. Acute-phase markers of inflammation and glomerular structure in patients with type 2 diabetes. J Am Soc Nephrol. 2005;16 Suppl 1:S78-82. [Crossref] [PubMed] 
  19. Kim AY, Kim HS, Kang JH, Yang MP. Serum adipokine concentrations in dogs with diabetes mellitus: a pilot study. J Vet Sci. 2015;16(3):333-40. [Crossref] [PubMed] [PMC] 
  20. Soetikno V, Sari FR, Veeraveedu PT, Thandavarayan RA, Harima M, Sukumaran V, et al. Curcumin ameliorates macrophage infiltration by inhibiting NF-κB activation and proinflammatory cytokines in streptozotocin induced-diabetic nephropathy. Nutr Metab (Lond). 2011;8(1):35. [PubMed] [PMC] 
  21. Zhang DW, Fu M, Gao SH, Liu JL. Curcumin and diabetes: a systematic review. Evid Based Complement Alternat Med. 2013;2013:636053. [PubMed] [PMC] 
  22. Kılıçlı MF, Acıbucu F. [Chronic induction, insulin resistance and diabetes]. Turkey Klinikleri J Pharmacol-Special Topics. 2015;3(3): 30-5. [Link] 
  23. Seo KI, Choi MS, Jung UJ, Kim HJ, Yeo J, Jeon SM, et al. Effect of curcumin supplementation on blood glucose, plasma insulin, and glucose homeostasis related enzyme activities in diabetic db/db mice. Mol Nutr Food Res. 2008;52(9):995-1004. [Crossref] [PubMed] 
  24. Patumraj S, Wongeakin N, Sridulyakul P, Jariyapongskul A, Futrakul N, Bunnag S. Combined effects of curcumin and vitamin C to protect endothelial dysfunction in the iris tissue of STZ-induced diabetic rats. Clin Hemorheol Microcirc. 2006;35(4):481-9. [PubMed] 
  25. Gutierres VO, Pinheiro CM, Assis RP, Vendramini RC, Pepato MT, Brunetti IL. Curcumin-supplemented yoghurt improves physiological and biochemical markers of experimental diabetes. Br J Nutr. 2012;108(3):440-8. [PubMed] 
  26. İslamoğlu Y, Koplay M, Sunay S, Açıkel M. [Obesity and metabolic syndrome]. Tıp Araştırmaları Dergisi. 2008;6(3):168-74. [Link] 
  27. El-Moselhy MA, Taye A, Sharkawi SS, El-Sisi SF, Ahmed AF. The antihyperglycemic effect of curcumin in high fat diet fed rats. Role of TNF-α and free fatty acids. Food Chem Toxicol. 2011;49(5):1129-40. [Crossref] [PubMed] 
  28. Li JM, Li YC, Kong LD, Hu QH. Curcumin inhibits hepatic protein-tyrosine phosphatase 1B and prevents hypertriglyceridemia and hepatic steatosis in fructose-fed rats. Hepatology. 2010;51(5):1555-66. [Crossref] [PubMed] 
  29. Naijil G, Anju TR, Jayanarayanan S, Paulose CS. Curcumin pretreatment mediates antidiabetogenesis via functional regulation of adrenergic receptor subtypes in the pancreas of multiple low-dose streptozotocin-induced diabetic rats. Nutr Res. 2015;35(9):823-33. [Crossref] [PubMed] 
  30. Kang C, Kim E. Synergistic effect of curcumin and insulin on muscle cell glucose metabolism. Food Chem Toxicol. 2010;48(8-9):2366-73. [PubMed] 
  31. Wickenberg J, Ingemansson SL, Hlebowicz J. Effects of Curcuma longa (turmeric) on postprandial plasma glucose and insulin in healthy subjects. Nutr J. 2010;9:43. [Crossref] [PubMed] [PMC] 
  32. Na LX, Zhang YL, Li Y, Liu LY, Li R, Kong T, et al. Curcumin improves insulin resistance in skeletal muscle of rats. Nutr Metab Cardiovasc Dis. 2011;21(7):526-33. [Crossref] [PubMed] 
  33. Song Z, Wang H, Zhu L, Han M, Gao Y, Du Y, et al. Curcumin improves high glucose-induced INS-1 cell insulin resistance via activation of insulin signaling. Food Funct. 2015;6(2):461-9. [Crossref] [PubMed] 
  34. Chougala MB, Bhaskar JJ, Rajan MG, Salimath PV. Effect of curcumin and quercetin on lysosomal enzyme activities in streptozotocin-induced diabetic rats. Clin Nutr. 2012;31(5):749-55. [Crossref] [PubMed] 
  35. Rashid K, Sil PC. Curcumin enhances recovery of pancreatic islets from cellular stress induced inflammation and apoptosis in diabetic rats. Toxicol Appl Pharmacol. 2015;282(3):297-310. [Crossref] [PubMed] 
  36. Kern PA, Ranganathan S, Li C, Wood L, Ranganathan G. Adipose tissue tumor necrosis factor and interleukin-6 expression in human obesity and insulin resistance. Am J Physiol Endocrinol Metab. 2001;280(5):E745-51. [Crossref] [PubMed] 
  37. Carey AL, Bruce CR, Sacchetti M, Anderson MJ, Olsen DB, Saltin B, et al. Interleukin-6 and tumor necrosis factor-alpha are not increased in patients with Type 2 diabetes: evidence that plasma interleukin-6 is related to fat mass and not insulin responsiveness. Diabetologia. 2004;47(6):1029-37. [Crossref] [PubMed] 

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